Flow cytometric analysis of ATG4C expression in HeLa cells using anti-ATG4C antibody (Cat #63403, 1:2,000). Green, isotype control; red, ATG4C.
Western blotting analysis using anti-ATG4C antibody (Cat#63403). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4C antibody (Cat#63403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ATG4C antibody (Cat#63403). ATG4C expression in wild-type (WT) and ATG4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG4C antibody (Cat#63403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Validation of ATG4C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ATG4C antibody (Cat#63403, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-ATG4C antibody (Cat#63403, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATG4C shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATG4C was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
