Flow cytometric analysis of erb-b2 receptor tyrosine kinase 2 expression in HepG2 cells using anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455, 1:2,000). Green, isotype control; red, erb-b2 receptor tyrosine kinase 2.
Immunocytochemical staining of HepG2 cells with anti-Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455, 1:1,000) . Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63455, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
