Western blotting analysis using anti-forkhead box P1 antibody (Cat#63461). Forkhead box P1 expression in wild-type (WT) and forkhead box P1 (FOXP1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-forkhead box P1 antibody (Cat#63461, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-forkhead box P1 antibody (Cat#63461). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-forkhead box P1 antibody (Cat#63461, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Forkhead box P1 expression in HAP-1 cells using anti-Forkhead box P1 antibody (Cat#63461, 1:2,000). Green, isotype control; red, Forkhead box P1.
Immunocytochemical staining of HAP-1 cells with Forkhead box P1 antibody (Cat#63461, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box P1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
