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KD-Validated Anti-RAD23B Mouse Monoclonal Antibody #63571

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Western blotting analysis using anti-RAD23B antibody (Cat#63571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RAD23B antibody (Cat#63571). RAD23B expression in wild type (WT) and RAD23B shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    RAD23B
  • 货号:
    63571
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-RAD23B Mouse Monoclonal Antibody
Aliases RAD23B; RAD23 Homolog B, Nucleotide Excision Repair Protein; HHR23B; HR23B; P58; XP-C Repair-Complementing Complex 58 KDa Protein; UV Excision Repair Protein RAD23 Homolog B; XP-C Repair Complementing Complex 58 KDa; XP-C Repair Complementing Protein; RAD23 (S. Cerevisiae) Homolog B; RAD23 Homolog B (S. Cerevisiae); RAD23, Yeast Homolog Of, B
Background

Gene Name: RAD23B

NCBI Gene Entry: 5887

UniProt Entry: P54727

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 53 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6575

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human RAD23B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-RAD23B antibody (Cat#63571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RAD23B antibody (Cat#63571). RAD23B expression in wild type (WT) and RAD23B shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-RAD23B antibody (Cat#63571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RAD23B antibody (Cat#63571). RAD23B expression in wild type (WT) and RAD23B shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAD23B antibody (Cat#63571, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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