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KD-Validated Anti-Granulin Precursor Mouse Monoclonal Antibody #63575

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Flow cytometric analysis of Granulin precursor expression in HepG2 cells using anti-Granulin precursor antibody (Cat#63575, 1:2,000). Green, isotype control; red, Granulin precursor.
Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Granulin precursor expression in wild type (WT) and granulin precursor (GRN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of GRN knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GRN antibody (Cat#63575, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    GRN
  • 货号：
    63575
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Granulin Precursor Mouse Monoclonal Antibody
Aliases GRN; Granulin Precursor; PCDGF; PGRN; Progranulin; CLN11; PC Cell-Derived Growth Factor; Glycoprotein Of 88 Kda; Glycoprotein 88; Proepithelin; Acrogranin; GP88; PEPI; Epithelin Precursor; Granulin-Epithelin; Epithelin; Granulins; GEP
Background

Gene Name: GRN

NCBI Gene Entry: 2896

UniProt Entry: P28799
Application Information

Molecular Weight: Predicted, 64 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6595

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human GRN
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Granulin precursor expression in HepG2 cells using anti-Granulin precursor antibody (Cat#63575, 1:2,000). Green, isotype control; red, Granulin precursor.
  • Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Granulin precursor expression in wild type (WT) and granulin precursor (GRN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of GRN knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GRN antibody (Cat#63575, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    GRN
  • 货号：
    63575
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Granulin precursor expression in HepG2 cells using anti-Granulin precursor antibody (Cat#63575, 1:2,000). Green, isotype control; red, Granulin precursor.
Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-granulin precursor antibody (Cat#63575). Granulin precursor expression in wild type (WT) and granulin precursor (GRN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of GRN knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GRN antibody (Cat#63575, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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