Western blotting analysis using anti-BH3 interacting domain death agonist antibody (Cat#63712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BH3 interacting domain death agonist antibody (Cat#63712, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-BH3 interacting domain death agonist antibody (Cat#63712). BH3 interacting domain death agonist expression in wild-type (WT) and BH3 interacting domain death agonist (BID) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BH3 interacting domain death agonist antibody (Cat#63712, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of BH3 interacting domain death agonist knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-BH3 interacting domain death agonist antibody (Cat#63712, 1:2,000) and analyzed using BD flow cytometer.
