Flow cytometric analysis of Transglutaminase 2 expression in HT-1080 cells using anti-Transglutaminase 2 antibody (Cat#63732, 1:2,000). Green, isotype control; red, Transglutaminase 2.
Western blotting analysis using anti-transglutaminase 2 antibody (Cat#63732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-transglutaminase 2 antibody (Cat#63732, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-transglutaminase 2 antibody (Cat#63732). Transglutaminase 2 expression in wild-type (WT) and transglutaminase 2 (TGM2) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-transglutaminase 2 antibody (Cat#63732, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Transglutaminase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-Transglutaminase 2 antibody (Cat#63732, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HT-1080 cells using anti-Transglutaminase 2 antibody (Cat#63732, 1:1,000), Top panel: wild-type (WT); Bottom panal:Transglutaminase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Transglutaminase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
