Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using anti-Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Green, isotype control; red, Small ubiquitin like modifier 1.
Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Small ubiquitin like modifier 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Small ubiquitin like modifier 1 antibody (Cat#63746, 1:1,000), Top panel: wild-type (WT); Bottom panal: Small ubiquitin like modifier 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Small ubiquitin like modifier 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
