Flow cytometric analysis of EZH2 expression in HAP-1 cells using anti-EZH2 antibody (Cat#63751, 1:2,000). Green, isotype control; red, EZH2.
Immunocytochemical staining of HAP-1 cells with anti-EZH2 antibody (Cat#63751, 1:1,000) . Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
Western blotting analysis using anti-EZH2 antibody (Cat#63751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EZH2 antibody (Cat#63751, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EZH2, enhancer of zeste 2 polycomb repressive complex 2 subunit.
Western blotting analysis using anti-EZH2 antibody (Cat#63751). EZH2 expression in wild-type (WT) and EZH2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EZH2 antibody (Cat#63751, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of EZH2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-EZH2 antibody (Cat#63751, 1:2,000) and analyzed using BD flow cytometer.
