Flow cytometric analysis of Lin-28 homolog B expression in HAP-1 cells using anti-Lin-28 homolog B antibody (Cat#63753, 1:2,000). Green, isotype control; red, Lin-28 homolog B.
Immunocytochemical staining of HAP1 cells with anti-Lin-28 homolog B antibody (Cat#63753, 1:1,000) . Nuclei were stained blue with DAPI;Lin-28 homolog B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-lin-28 homolog B antibody (Cat#63753). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lin-28 homolog B antibody (Cat#63753, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-lin-28 homolog B antibody (Cat#63753). Lin-28 homolog B expression in wild-type (WT) and lin-28 homolog B (LIN28B) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lin-28 homolog B antibody (Cat#63753, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Lin-28 homolog B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Lin-28 homolog B antibody (Cat#63753, 1:2,000) and analyzed using BD flow cytometer.
