Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:1,000). Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.
Western blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). 3-hydroxybutyrate dehydrogenase 2 expression in wild type (WT) and 3-hydroxybutyrate dehydrogenase 2 (BDH2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
