Flow cytometric analysis of Crystallin zeta like 1 expression in HeLa cells using anti-Crystallin zeta like 1 antibody (Cat#63805, 1:1,000). Green, isotype control; red, Crystallin zeta like 1.
Western blotting analysis using anti-crystallin zeta like 1 antibody (Cat#63805). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-crystallin zeta like 1 antibody (Cat#63805). Crystallin zeta like 1 expression in wild-type (WT) and crystallin zeta like 1 (CRYZL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
