Flow cytometric analysis of Coiled-coil domain containing 93 expression in HeLa cells using anti-Coiled-coil domain containing 93 antibody (Cat#63810, 1:1,000). Green, isotype control; red, Coiled-coil domain containing 93.
Western blotting analysis using anti-coiled-coil domain containing 93 antibody (Cat#63810). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-coiled-coil domain containing 93 antibody (Cat#63810, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-coiled-coil domain containing 93 antibody (Cat#63810). Coiled-coil domain containing 93 expression in wild type (WT) and coiled-coil domain containing 93 (CCDC93) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-coiled-coil domain containing 93 antibody (Cat#63810, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).