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KD-Validated Anti-AAAS Mouse Monoclonal Antibody #63820

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Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000). Green, isotype control; red, Aladin WD repeat nucleoporin.
Immunocytochemical staining of HAP1 cells with anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:500) . Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Aladin WD repeat nucleoporin expression in wild type (WT) and aladin WD repeat nucleoporin (AAAS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of AAAS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-AAAS antibody (Cat#63820, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000), Top panel: wild-type (WT); Bottom panal: Aladin WD repeat nucleoporin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    AAAS
  • 货号：
    63820
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-AAAS Mouse Monoclonal Antibody
Aliases AAAS; Aladin WD Repeat Nucleoporin; Adracalin; Aladin; Achalasia, Adrenocortical Insufficiency, Alacrimia; Allgrove, Triple-A; ADRACALA; Achalasia, Adrenocortical Insufficiency, Alacrimia (Allgrove, Triple-A); AAASb; GL003; AAA
Background

Gene Name: AAAS

NCBI Gene Entry: 8086

UniProt Entry: Q9NRG9
Application Information

Molecular Weight: Predicted, 60 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8035

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human AAAS
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000). Green, isotype control; red, Aladin WD repeat nucleoporin.
  • Immunocytochemical staining of HAP1 cells with anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:500) . Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Aladin WD repeat nucleoporin expression in wild type (WT) and aladin WD repeat nucleoporin (AAAS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of AAAS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-AAAS antibody (Cat#63820, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000), Top panel: wild-type (WT); Bottom panal: Aladin WD repeat nucleoporin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    AAAS
  • 货号：
    63820
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000). Green, isotype control; red, Aladin WD repeat nucleoporin.
Immunocytochemical staining of HAP1 cells with anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:500) . Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-aladin WD repeat nucleoporin antibody (Cat#63820). Aladin WD repeat nucleoporin expression in wild type (WT) and aladin WD repeat nucleoporin (AAAS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-aladin WD repeat nucleoporin antibody (Cat#63820, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of AAAS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-AAAS antibody (Cat#63820, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Aladin WD repeat nucleoporin antibody (Cat#63820, 1:1,000), Top panel: wild-type (WT); Bottom panal: Aladin WD repeat nucleoporin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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