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KD-Validated Anti-HIBCH Mouse Monoclonal Antibody #64808

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Flow cytometric analysis of HIBCH expression in HepG2 cells using anti-HIBCH antibody (Cat#64808, 1:2,000). Green, isotype control; red, HIBCH.

Western blotting analysis using anti-HIBCH antibody (Cat#64808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIBCH antibody (Cat#64808, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-HIBCH antibody (Cat#64808). HIBCH expression in wild-type (WT) and HIBCH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIBCH antibody (Cat#64808, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of HIBCH knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HIBCH antibody (Cat#64808, 1:2,000) and analyzed using BD flow cytometer.

基因名：

HIBCH
货号：

64808
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-HIBCH Mouse Monoclonal Antibody
Aliases	HIBCH; 3-Hydroxyisobutyryl-CoAHydrolase; 3-Hydroxyisobutyryl-Coenzyme A Hydrolase; 3-Hydroxyisobutyryl-CoA Hydrolase, Mitochondrial; HIB-CoA Hydrolase; HIBYL-CoA-H; EC 3.1.2.4; Testicular Tissue Protein Li 86; HIBYLCOAH
Background	Gene Name: HIBCH NCBI Gene Entry: 26275 UniProt Entry: Q6NVY1
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 37 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB13045 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human HIBCH
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of HIBCH expression in HepG2 cells using anti-HIBCH antibody (Cat#64808, 1:2,000). Green, isotype control; red, HIBCH.
Western blotting analysis using anti-HIBCH antibody (Cat#64808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIBCH antibody (Cat#64808, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HIBCH antibody (Cat#64808). HIBCH expression in wild-type (WT) and HIBCH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIBCH antibody (Cat#64808, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of HIBCH knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HIBCH antibody (Cat#64808, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

HIBCH
货号：

64808
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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