Flow cytometric analysis of FUBP3 expression in HT-1080 cells using anti-FUBP3 antibody (Cat#64829, 1:2,000). Green, isotype control; red, FUBP3.
Immunocytochemical staining of HT-1080 cells with anti-FUBP3 associated protein antibody (Cat#64829, 1:1,000) . Nuclei were stained blue with DAPI; FUBP3 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FUBP3 antibody (Cat#64829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FUBP3 antibody (Cat#64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-FUBP3 antibody (Cat #64829). FUBP3 expression in wild-type (WT) and FUBP3 shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FUBP3 antibody (Cat #64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
