Flow cytometric analysis of PAICS expression in HAP-1 cells using anti-PAICS antibody (Cat#64841, 1:2,000). Green, isotype control; red, PAICS.
Western blotting analysis using anti-PAICS antibody (Cat#64841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PAICS antibody (Cat#64841). PAICS expression in wild-type (WT) and PAICS shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PAICS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PAICS antibody (Cat#64841, 1:2,000) and analyzed using BD flow cytometer.