Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using anti-Sirtuin 5 antibody (Cat#64846, 1:2,000). Green, isotype control; red, Sirtuin 5.
Immunocytochemical staining of HepG2 cells with anti-Sirtuin 5 associated protein antibody (Cat#64846, 1:1,000) . Nuclei were stained blue with DAPI; Sirtuin 5 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-sirtuin 5 antibody (Cat#64846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sirtuin 5 antibody (Cat#64846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-sirtuin 5 antibody (Cat#64846). Sirtuin 5 expression in wild-type (WT) and sirtuin 5 (SIRT5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-sirtuin 5 antibody (Cat#64846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
