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KD-Validated Anti-WD Repeat Domain 77 Mouse Monoclonal Antibody #64871

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Flow cytometric analysis of WDR77 expression in HeLa cells using anti-WDR77 antibody (Cat#64871, 1:2,000). Green, isotype control; red, WDR77.
Immunocytochemical staining of Hela cells with anti-WDR77 associated protein antibody (Cat#64871, 1:1,000) . Nuclei were stained blue with DAPI; WDR77 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-WDR77 antibody (Cat#64871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-WDR77 antibody (Cat#64871). WDR77 expression in wild-type (WT) and WDR77 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of WDR77 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-WDR77 antibody (Cat#64871, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-WDR77 antibody (Cat#64871, 1:1,000), Top panel: wild-type (WT); Bottom panal: WDR77 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名:
    WDR77
  • 货号:
    64871
  • 应用:
    WB, IC, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-WD Repeat Domain 77 Mouse Monoclonal Antibody
Aliases WDR77; WD Repeat Domain 77; MEP50; Methylosome Protein 50; P44; WD Repeat-Containing Protein 77; Androgen Receptor Cofactor P44; Methylosome Protein WDR77; P44/Mep50; MEP-50; Testis Tissue Sperm-Binding Protein Li 44a; Nbla10071; HKMT1069; WD45
Background

Gene Name: WDR77

NCBI Gene Entry: 79084

UniProt Entry: Q9BQA1

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 42 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13360

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human WDR77
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of WDR77 expression in HeLa cells using anti-WDR77 antibody (Cat#64871, 1:2,000). Green, isotype control; red, WDR77.
  • Immunocytochemical staining of Hela cells with anti-WDR77 associated protein antibody (Cat#64871, 1:1,000) . Nuclei were stained blue with DAPI; WDR77 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-WDR77 antibody (Cat#64871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-WDR77 antibody (Cat#64871). WDR77 expression in wild-type (WT) and WDR77 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of WDR77 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-WDR77 antibody (Cat#64871, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-WDR77 antibody (Cat#64871, 1:1,000), Top panel: wild-type (WT); Bottom panal: WDR77 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
Flow cytometric analysis of WDR77 expression in HeLa cells using anti-WDR77 antibody (Cat#64871, 1:2,000). Green, isotype control; red, WDR77.
Immunocytochemical staining of Hela cells with anti-WDR77 associated protein antibody (Cat#64871, 1:1,000) . Nuclei were stained blue with DAPI; WDR77 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-WDR77 antibody (Cat#64871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-WDR77 antibody (Cat#64871). WDR77 expression in wild-type (WT) and WDR77 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WDR77 antibody (Cat#64871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of WDR77 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-WDR77 antibody (Cat#64871, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-WDR77 antibody (Cat#64871, 1:1,000), Top panel: wild-type (WT); Bottom panal: WDR77 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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