Flow cytometric analysis of PPP4C expression in HAP-1 cells using anti-PPP4C antibody (Cat#64872, 1:2,000). Green, isotype control; red, PPP4C.
Immunocytochemical staining of HAP-1 cells with anti-PPP4C antibody (Cat#64872, 1:1,000) . Nuclei were stained blue with DAPI; PPP4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-PPP4C antibody (Cat#64872). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP4C antibody (Cat#64872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PPP4C antibody (Cat#64872). PPP4C expression in wild-type (WT) and PPP4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP4C antibody (Cat#64872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
