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KD-Validated Anti-NEDD4 Mouse Monoclonal Antibody #64880

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Western blotting analysis using anti-NEDD4 antibody (Cat#64880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-NEDD4 antibody (Cat#64880). NEDD4 expression in wild-type (WT) and NEDD4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NEDD4
货号：

64880
应用：

WB
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-NEDD4 Mouse Monoclonal Antibody
Aliases	NEDD4; NEDD4 E3 Ubiquitin Protein Ligase; NEDD4-1; RPF1; KIAA0093; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase; Cell Proliferation-Inducing Gene 53 Protein; HECT-Type E3 Ubiquitin Transferase NEDD4; E3 Ubiquitin-Protein Ligase NEDD4; Receptor-Potentiating Factor 1; MGC176705; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 4; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4; EC 2.3.2.26; EC 6.3.2; NEDD-4
Background	Gene Name: NEDD4 NCBI Gene Entry: 4734 UniProt Entry: P46934
Application Information	Molecular Weight: Predicted, 149 kDa; observed, 130 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB15775 Species Reactivity: Human Applications Tested: Western blotting (WB)
Immunogen	Recombinant protein of human NEDD4
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500
Note	This product is for research use only.

Picture

Western blotting analysis using anti-NEDD4 antibody (Cat#64880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NEDD4 antibody (Cat#64880). NEDD4 expression in wild-type (WT) and NEDD4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NEDD4
货号：

64880
应用：

WB
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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