Flow cytometric analysis of CD168 expression in HAP-1 cells using anti-CD168 antibody (Cat#64888, 1:2,000). Green, isotype control; red, CD168.
Immunocytochemical staining of HAP-1 cells with anti-CD168 antibody (Cat#64888, 1:1,000) . Nuclei were stained blue with DAPI; CD168 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-CD168 antibody (Cat#64888). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD168 antibody (Cat#64888, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CD168 antibody (Cat#64888). CD168 expression in wild-type (WT) and HMMR knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CD168 antibody (Cat#64888, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
