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KD-Validated Anti-HNRNPUL1 Rabbit Monoclonal Antibody #64893

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Flow cytometric analysis of HNRNPUL1 expression in HAP-1 cells using anti-HNRNPUL1 antibody (Cat#64893, 1:2,000). Green, isotype control; red, HNRNPUL1.
Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). HNRNPUL1 expression in wild-type (WT) and HNRNPUL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of HNRNPUL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HNRNPUL1 antibody (Cat#64893, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-HNRNPUL1 antibody (Cat#64893, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    HNRNPUL1
  • 货号：
    64893
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-HNRNPUL1 Rabbit Monoclonal Antibody
Aliases HNRNPUL1; Heterogeneous Nuclear Ribonucleoprotein U Like 1; E1B-AP5; E1BAP5; HNRPUL1; Heterogeneous Nuclear Ribonucleoprotein U-Like Protein 1; Adenovirus Early Region 1B-Associated Protein 5; E1B-55 KDa-Associated Protein 5; E1B 55kDa Associated Protein 5; FLJ12944
Background

Gene Name: HNRNPUL1

NCBI Gene Entry: 11100

UniProt Entry: Q9BUJ2
Application Information

Molecular Weight: Predicted, 96 kDa; observed, 90-125 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13625

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human E1B-AP5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM) : 1:2,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HNRNPUL1 expression in HAP-1 cells using anti-HNRNPUL1 antibody (Cat#64893, 1:2,000). Green, isotype control; red, HNRNPUL1.
  • Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). HNRNPUL1 expression in wild-type (WT) and HNRNPUL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of HNRNPUL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HNRNPUL1 antibody (Cat#64893, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-HNRNPUL1 antibody (Cat#64893, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    HNRNPUL1
  • 货号：
    64893
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of HNRNPUL1 expression in HAP-1 cells using anti-HNRNPUL1 antibody (Cat#64893, 1:2,000). Green, isotype control; red, HNRNPUL1.
Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HNRNPUL1 antibody (Cat#64893). HNRNPUL1 expression in wild-type (WT) and HNRNPUL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPUL1 antibody (Cat#64893, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of HNRNPUL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HNRNPUL1 antibody (Cat#64893, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HAP-1 cells with anti-HNRNPUL1 antibody (Cat#64893, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPUL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-HNRNPUL1 antibody (Cat#64893, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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