Western blotting analysis using anti-annexin A5 antibody (Cat#64994). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A5 antibody (Cat#64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-annexin A5 antibody (Cat #64994). Annexin A5 expression in wild-type (WT) and annexin A5 (ANXA5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A5 antibody (Cat #64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
A synthesized peptide derived from human Annexin A5
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Picture
Western blotting analysis using anti-annexin A5 antibody (Cat#64994). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A5 antibody (Cat#64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-annexin A5 antibody (Cat #64994). Annexin A5 expression in wild-type (WT) and annexin A5 (ANXA5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A5 antibody (Cat #64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Western blotting analysis using anti-annexin A5 antibody (Cat#64994). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A5 antibody (Cat#64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-annexin A5 antibody (Cat #64994). Annexin A5 expression in wild-type (WT) and annexin A5 (ANXA5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A5 antibody (Cat #64994, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
