KD-Validated Anti-SH3GL1 Mouse Monoclonal Antibody - 产品中心

KD-Validated Anti-SH3GL1 Mouse Monoclonal Antibody #65029

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Western blotting analysis using anti-SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of SH3GL1 expression in HepG2 cells using anti-SH3GL1 antibody (Cat#65029, 1:1,000). Green, isotype control; red, SH3GL1.

基因名：

SH3GL1
货号：

65029
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

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Citation(0)

Information

Product Name	KD-Validated Anti-SH3GL1 Mouse Monoclonal Antibody
Aliases	SH3GL1; SH3 Domain Containing GRB2 Like 1, Endophilin A2; SH3D2B; CNSA1; EEN; SH3P8; Extra Eleven-Nineteen Leukemia Fusion Gene Protein; SH3 Domain-Containing GRB2-Like Protein 1; SH3-Containing Grb-2-Like 1 Protein; Extra 11-19 Leukemia Fusion; EEN Fusion Partner Of MLL; SH3-Domain GRB2-Like 1; SH3 Domain Protein 2B; Endophilin-A2; Endophilin-2; MGC111371; SH3 Domain Containing GRB2 Like 1; SH3-Containing Protein EEN; SH3 Domain GRB2-Like 1; Fusion Partner Of MLL; Endophilin A2
Background	Gene Name: SH3GL1 NCBI Gene Entry: 6455 UniProt Entry: Q99961
Application Information	Molecular Weight: Predicted, 41 kDa; observed, 45 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB13930 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human SH3GL1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:1,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer.
Flow cytometric analysis of SH3GL1 expression in HepG2 cells using anti-SH3GL1 antibody (Cat#65029, 1:1,000). Green, isotype control; red, SH3GL1.

Citation(0)

基因名：

SH3GL1
货号：

65029
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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