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KD-Validated Anti-PPM1B Mouse Monoclonal Antibody #65037

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Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-PPM1B antibody (Cat#65037, 1:1,000) . Nuclei were stained blue with DAPI; PPM1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPM1B expression in HepG2 cells using anti-PPM1B antibody (Cat#65037, 1:1,000). Green, isotype control; red, PPM1B.
  • 基因名：
    PPM1B
  • 货号：
    65037
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PPM1B Mouse Monoclonal Antibody
Aliases PPM1B; Protein Phosphatase, Mg2+/Mn2+ Dependent 1B; PP2CB; PPC2BETAX; PP2CBETA; Protein Phosphatase 1B; EC 3.1.3.16; PP2C-Beta; Protein Phosphatase 1B (Formerly 2C), Magnesium-Dependent, Beta Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1B; Protein Phosphatase 2C, Beta Isoform; Protein Phosphatase 2C-Like Protein; Protein Phosphatase 2C Isoform Beta; PP2C-Beta-X
Background

Gene Name: PPM1B

NCBI Gene Entry: 5495

UniProt Entry: O75688
Application Information

Molecular Weight: Predicted, 53 kDa; observed, 39,53 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13970

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human PPM1B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-PPM1B antibody (Cat#65037, 1:1,000) . Nuclei were stained blue with DAPI; PPM1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PPM1B expression in HepG2 cells using anti-PPM1B antibody (Cat#65037, 1:1,000). Green, isotype control; red, PPM1B.
Citation(0)
  • 基因名：
    PPM1B
  • 货号：
    65037
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-PPM1B antibody (Cat#65037, 1:1,000) . Nuclei were stained blue with DAPI; PPM1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPM1B expression in HepG2 cells using anti-PPM1B antibody (Cat#65037, 1:1,000). Green, isotype control; red, PPM1B.
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