Western blotting analysis using anti-centrosomal protein 55 antibody (Cat#65058). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-centrosomal protein 55 antibody (Cat#65058). Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using anti-Centrosomal protein 55 antibody (Cat#65058, 1:1,000). Green, isotype control; red, Centrosomal protein 55.
