Western blotting analysis using anti-IKBKG antibody (Cat#65173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-IKBKG antibody (Cat#65173). IKBKG expression in wild-type (WT) and IKBKG knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IKBKG expression in HeLa cells using anti-IKBKG antibody (Cat#65173, 1:1,000). Green, isotype control; red, IKBKG.