Western blotting analysis using anti-REEP2 antibody (Cat#65174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-REEP2 antibody (Cat#65174). REEP2 expression in wild-type (WT) and REEP2 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of REEP2 expression in HeLa cells using anti-REEP2 antibody (Cat#65174, 1:1,000). Green, isotype control; red, REEP2.