Flow cytometric analysis of Cyclin T1 expression in HeLa cells using anti-Cyclin T1 antibody (Cat#65205, 1:2,000). Green, isotype control; red, Cyclin T1.
Immunocytochemical staining of Hela cells with anti-Cyclin T1 protein antibody (Cat#65205, 1:1,000) . Nuclei were stained blue with DAPI; Cyclin T1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cyclin T1 antibody (Cat#65205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin T1 antibody (Cat#65205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cyclin T1 antibody (Cat#65205). Cyclin T1 expression in wild-type (WT) and cyclin T1 (CCNT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin T1 antibody (Cat#65205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
