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KD-Validated Anti-KDM2A Rabbit Monoclonal Antibody #65207

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Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using anti-Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    KDM2A
  • 货号：
    65207
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-KDM2A Rabbit Monoclonal Antibody
Aliases KDM2A; Lysine Demethylase 2A; JHDM1A; FBL11; CXXC8; FBL7; F-Box And Leucine-Rich Repeat Protein 11; KIAA1004; LILINA; FBXL11; JmjC Domain-Containing Histone Demethylation Protein 1A; Jumonji C Domain-Containing Histone Demethylase 1A; [Histone-H3]-Lysine-36 Demethylase 1A; Lysine (K)-Specific Demethylase 2A; CXXC-Type Zinc Finger Protein 8; Lysine-Specific Demethylase 2A; F-Box/LRR-Repeat Protein 11; DKFZP434M1735; EC 1.14.11.27; FLJ00115; F-Box Protein Lilina; F-Box Protein FBL11; F-Box Protein FBL7
Background

Gene Name: KDM2A

NCBI Gene Entry: 22992

UniProt Entry: Q9Y2K7
Application Information

Molecular Weight: Predicted, 133 kDa; observed, 133 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB160

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human FBXL11
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using anti-Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
  • Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    KDM2A
  • 货号：
    65207
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using anti-Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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