KD-Validated Anti-MTHFR Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-MTHFR Rabbit Monoclonal Antibody #65213

一键复制产品信息

返回列表

Flow cytometric analysis of MTHFR expression in HeLa cells using anti-MTHFR antibody (Cat#65213, 1:2,000). Green, isotype control; red, MTHFR.

Western blotting analysis using anti-MTHFR antibody (Cat#65213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-MTHFR antibody (Cat#65213). MTHFR expression in wild-type (WT) and MTHFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MTHFR
货号：

65213
应用：

WB, FCM
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-MTHFR Rabbit Monoclonal Antibody
Aliases	MTHFR; Methylenetetrahydrofolate Reductase; 5,10-Methylenetetrahydrofolate Reductase (NADPH); Methylenetetrahydrofolate Reductase (NAD(P)H); Methylenetetrahydrofolate Reductase (NADPH); EC 1.5.1.53; EC 1.5.1.20
Background	Gene Name: MTHFR NCBI Gene Entry: 4524 UniProt Entry: P42898
Application Information	Molecular Weight: Predicted, 75 kDa; observed, 70 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB190 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human MTHFR
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of MTHFR expression in HeLa cells using anti-MTHFR antibody (Cat#65213, 1:2,000). Green, isotype control; red, MTHFR.
Western blotting analysis using anti-MTHFR antibody (Cat#65213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-MTHFR antibody (Cat#65213). MTHFR expression in wild-type (WT) and MTHFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MTHFR
货号：

65213
应用：

WB, FCM
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页