KD-Validated Anti-RAP1B Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-RAP1B Rabbit Monoclonal Antibody #65217

一键复制产品信息

返回列表

Flow cytometric analysis of RAP1B expression in HepG2 cells using anti-RAP1B antibody (Cat#65217, 1:2,000). Green, isotype control; red, RAP1B.

Immunocytochemical staining of HepG2 cells with anti-RAP1B antibody (Cat#65217, 1:1,000) . Nuclei were stained blue with DAPI; RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-RAP1B antibody (Cat#65217). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-RAP1B antibody (Cat#65217). RAP1B expression in wild-type (WT) and RAP1B shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

RAP1B
货号：

65217
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-RAP1B Rabbit Monoclonal Antibody
Aliases	RAP1B; RAP1B, Member Of RAS Oncogene Family; K-REV; RAL1B; GTP-Binding Protein Smg P21B; Ras-Related Protein Rap-1b; DKFZp586H0723; Ras Family Small GTP Binding Protein RAP1B; RAS-Related Protein RAP1B; Small GTP Binding Protein; EC 3.6.5.2; THC11
Background	Gene Name: RAP1B NCBI Gene Entry: 5908 UniProt Entry: P61224
Application Information	Molecular Weight: Predicted, 21 kDa; observed, 20 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB210 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RAP1A/B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RAP1B expression in HepG2 cells using anti-RAP1B antibody (Cat#65217, 1:2,000). Green, isotype control; red, RAP1B.
Immunocytochemical staining of HepG2 cells with anti-RAP1B antibody (Cat#65217, 1:1,000) . Nuclei were stained blue with DAPI; RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RAP1B antibody (Cat#65217). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-RAP1B antibody (Cat#65217). RAP1B expression in wild-type (WT) and RAP1B shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

RAP1B
货号：

65217
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页