Western blotting analysis using anti-TFAP2C antibody (Cat#65311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TFAP2C antibody (Cat#65311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TFAP2C antibody (Cat#65311). TFAP2C expression in wild-type (WT) and TFAP2C shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TFAP2C antibody (Cat#65311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TFAP2C expression in HAP-1 cells using anti-TFAP2C antibody (Cat#65311, 1:2,000). Green, isotype control; red, TFAP2C.