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KD-Validated Anti-H1-3 Rabbit Monoclonal Antibody #65317

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Western blotting analysis using anti-H1-3 antibody (Cat#65317). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-H1-3 antibody (Cat#65317). H1-3 expression in wild-type (WT) and H1-3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    H1-3
  • 货号:
    65317
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-H1-3 Rabbit Monoclonal Antibody
Aliases H1-3; H1.3 Linker Histone, Cluster Member; HIST1H1D; H1s-2; H1.3; H1F3; H1d; Histone Cluster 1 H1 Family Member D; H1 Histone Family, Member 3; Histone Cluster 1, H1d; Histone 1, H1d; Histone H1s-2 ;Histone H1.3; Histone H1c
Background

Gene Name: H1-3

NCBI Gene Entry: 3007

UniProt Entry: P16402

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 31 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB915

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Histone H1.3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-H1-3 antibody (Cat#65317). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-H1-3 antibody (Cat#65317). H1-3 expression in wild-type (WT) and H1-3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-H1-3 antibody (Cat#65317). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-H1-3 antibody (Cat#65317). H1-3 expression in wild-type (WT) and H1-3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-H1-3 antibody (Cat#65317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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