KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody #65322

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Western blotting analysis using anti-UBE2F antibody (Cat#65322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-UBE2F antibody (Cat#65322). UBE2F expression in wild-type (WT) and UBE2F shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of UBE2F expression in HAP-1 cells using anti-UBE2F antibody (Cat#65322, 1:2,000). Green, isotype control; red, UBE2F.

基因名：

UBE2F
货号：

65322
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody
Aliases	UBE2F; Ubiquitin Conjugating Enzyme E2 F (Putative); NCE2; Ubiquitin-Conjugating Enzyme E2F (Putative); RING-Type E3 NEDD8 Transferase UBE2F; NEDD8-Conjugating Enzyme UBE2F; NEDD8 Carrier Protein UBE2F; NEDD8 Protein Ligase UBE2F; NEDD8-Conjugating Enzyme 2; Ubiquitin-Conjugating Enzyme E2 F; NEDD8 Conjugating Enzyme; EC 2.3.2.34; EC 6.3.2
Background	Gene Name: UBE2F NCBI Gene Entry: 140739 UniProt Entry: UniProt Entry: Q969M7
Application Information	Molecular Weight: Predicted, 21 kDa; observed, 20 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB940 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human UBE2F
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-UBE2F antibody (Cat#65322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-UBE2F antibody (Cat#65322). UBE2F expression in wild-type (WT) and UBE2F shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of UBE2F expression in HAP-1 cells using anti-UBE2F antibody (Cat#65322, 1:2,000). Green, isotype control; red, UBE2F.

Citation(0)

基因名：

UBE2F
货号：

65322
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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