Flow cytometric analysis of FNTA expression in HepG2 cells using anti-FNTA antibody (Cat#65441, 1,2000). Green, isotype control; red, FNTA.
Western blotting analysis using anti-FNTA antibody (Cat#65441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FNTA antibody (Cat#65441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-FNTA antibody (Cat#65441). FNTA expression in wild-type (WT) and FNTA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FNTA antibody (Cat#65441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of FNTA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-FNTA antibody (Cat#65441, 1:2,000) and analyzed using BD flow cytometer.
