Western blotting analysis using anti-cathepsin Z antibody (Cat#65559). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cathepsin Z antibody (Cat#65559, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cathepsin Z antibody (Cat#65559). Cathepsin Z expression in wild-type (WT) and cathepsin Z (CTSZ) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cathepsin Z antibody (Cat#65559, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Cathepsin Z expression in HT-1080 cells using anti-Cathepsin Z antibody (Cat#65559, 1:2,000). Green, isotype control; red, Cathepsin Z.