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KD-Validated Anti-Cullin 5 Rabbit Monoclonal Antibody #65564

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Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-Cullin 5 antibody (Cat#65564, 1:1,000) . Nuclei were stained blue with DAPI; Cullin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Cullin 5 expression in C2C12 cells using anti-Cullin 5 antibody (Cat#65564, 1:2,000). Green, isotype control; red, Cullin 5.
  • 基因名：
    CUL5
  • 货号：
    65564
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Cullin 5 Rabbit Monoclonal Antibody
Aliases CUL5; Cullin 5; VACM-1; Vasopressin-Activated Calcium-Mobilizing Receptor 1; Cullin-5; CUL-5; VACM1; Cullin-5 (Vasopressin-Activated Calcium-Mobilizing Receptor-1); Vasopressin-Activated Calcium-Mobilizing Receptor-1
Background

Gene Name: CUL5

NCBI Gene Entry: 8065

UniProt Entry: Q93034
Application Information

Molecular Weight: Predicted, 91 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4280

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human Cullin 5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with anti-Cullin 5 antibody (Cat#65564, 1:1,000) . Nuclei were stained blue with DAPI; Cullin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Cullin 5 expression in C2C12 cells using anti-Cullin 5 antibody (Cat#65564, 1:2,000). Green, isotype control; red, Cullin 5.
Citation(0)
  • 基因名：
    CUL5
  • 货号：
    65564
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-Cullin 5 antibody (Cat#65564, 1:1,000) . Nuclei were stained blue with DAPI; Cullin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cullin 5 antibody (Cat#65564). Cullin 5 expression in wild-type (WT) and cullin 5 (CUL5) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cullin 5 antibody (Cat#65564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Cullin 5 expression in C2C12 cells using anti-Cullin 5 antibody (Cat#65564, 1:2,000). Green, isotype control; red, Cullin 5.
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