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KD-Validated Anti-MAPK6 Rabbit Monoclonal Antibody #65580

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Western blotting analysis using anti-MAPK6 antibody (Cat#65580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-MAPK6 antibody (Cat#65580). MAPK6 expression in wild-type (WT) and MAPK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of MAPK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MAPK6 antibody (Cat#65580, 1:2,000) and analyzed using BD flow cytometer.

基因名：

MAPK6
货号：

65580
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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Information

Product Name	KD-Validated Anti-MAPK6 Rabbit Monoclonal Antibody
Aliases	MAPK6; Mitogen-Activated Protein Kinase 6; ERK3; HsT17250; P97MAPK; PRKM6; Extracellular Signal-Regulated Kinase 3; MAP Kinase 6; EC 2.7.11.24; P97-MAPK; MAPK 6; ERK-3; Extracellular Signal-Regulated Kinase, P97; Protein Kinase, Mitogen-Activated 5; Protein Kinase, Mitogen-Activated 6; MAP Kinase Isoform P97; EC 2.7.11
Background	Gene Name: MAPK6 NCBI Gene Entry: 5597 UniProt Entry: Q16659
Application Information	Molecular Weight: Predicted, 83 kDa; observed, 105 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4360 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human MAPK6
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-MAPK6 antibody (Cat#65580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MAPK6 antibody (Cat#65580). MAPK6 expression in wild-type (WT) and MAPK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAPK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MAPK6 antibody (Cat#65580, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

MAPK6
货号：

65580
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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