Western blotting analysis using anti-RAB10 antibody (Cat#65596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-RAB10 antibody (Cat#65596). RAB10 expression in wild-type (WT) and RAB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RAB10 expression in H9c2 cells using anti-RAB10 antibody (Cat#65596, 1:2,000). Green, isotype control; red, RAB10.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human RAB10
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-RAB10 antibody (Cat#65596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-RAB10 antibody (Cat#65596). RAB10 expression in wild-type (WT) and RAB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RAB10 expression in H9c2 cells using anti-RAB10 antibody (Cat#65596, 1:2,000). Green, isotype control; red, RAB10.
Western blotting analysis using anti-RAB10 antibody (Cat#65596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-RAB10 antibody (Cat#65596). RAB10 expression in wild-type (WT) and RAB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAB10 antibody (Cat#65596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RAB10 expression in H9c2 cells using anti-RAB10 antibody (Cat#65596, 1:2,000). Green, isotype control; red, RAB10.
