Flow cytometric analysis of Pyridoxal kinase expression in HepG2 cells using anti-Pyridoxal kinase antibody (Cat#65849, 1:2,000). Green, isotype control; red, Pyridoxal kinase.
Western blotting analysis using anti-pyridoxal kinase antibody (Cat#65849). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pyridoxal kinase antibody (Cat#65849, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-pyridoxal kinase antibody (Cat#65849). Pyridoxal kinase expression in wild-type (WT) and pyridoxal kinase (PDXK) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pyridoxal kinase antibody (Cat#65849, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
