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KD-Validated Anti-NIPSNAP2 Mouse Monoclonal Antibody #65866

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Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of NIPSNAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) NIPSNAP2 cells were stained with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and analyzed using BD flow cytometer.
Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-NIPSNAP2 antibody (Cat#65866, 1:1,000), Top panel: wild-type (WT); Bottom panal: NIPSNAP2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    NIPSNAP2
  • 货号：
    65866
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-NIPSNAP2 Mouse Monoclonal Antibody
Aliases NIPSNAP2; Nipsnap Homolog 2; GBAS; Glioblastoma Amplified Sequence; Protein NipSnap Homolog 2; 4-Nitrophenylphosphatase Domain And Non-Neuronal SNAP25-Like 2; Glioblastoma-Amplified Sequence ; NipSnap2
Background

Gene Name: NIPSNAP2

NCBI Gene Entry: 2631

UniProt Entry: O75323
Application Information

Molecular Weight: Predicted, 34 kDa, observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5520

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human GBAS
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
  • Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of NIPSNAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) NIPSNAP2 cells were stained with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and analyzed using BD flow cytometer.
  • Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
  • Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using anti-NIPSNAP2 antibody (Cat#65866, 1:1,000), Top panel: wild-type (WT); Bottom panal: NIPSNAP2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    NIPSNAP2
  • 货号：
    65866
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of NIPSNAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) NIPSNAP2 cells were stained with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and analyzed using BD flow cytometer.
Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using anti-NIPSNAP2 antibody (Cat#65866, 1:2,000). Green, isotype control; red, NIPSNAP2.
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NIPSNAP2 antibody (Cat#65866). NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIPSNAP2 antibody (Cat#65866, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-NIPSNAP2 antibody (Cat#65866, 1:1,000), Top panel: wild-type (WT); Bottom panal: NIPSNAP2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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