Western blotting analysis using anti-VRK1 antibody (Cat#65871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-VRK1 antibody (Cat#65871). VRK1 expression in wild-type (WT) and VRK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VRK1 expression in HAP-1 cells using anti-VRK1 antibody (Cat#65871, 1:2,000). Green, isotype control; red, VRK1.
Western blotting analysis using anti-VRK1 antibody (Cat#65871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-VRK1 antibody (Cat#65871). VRK1 expression in wild-type (WT) and VRK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VRK1 expression in HAP-1 cells using anti-VRK1 antibody (Cat#65871, 1:2,000). Green, isotype control; red, VRK1.
Western blotting analysis using anti-VRK1 antibody (Cat#65871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-VRK1 antibody (Cat#65871). VRK1 expression in wild-type (WT) and VRK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-VRK1 antibody (Cat#65871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VRK1 expression in HAP-1 cells using anti-VRK1 antibody (Cat#65871, 1:2,000). Green, isotype control; red, VRK1.