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KD-Validated Anti-TANK Mouse Monoclonal Antibody #65874

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Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of TANK knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TANK antibody (Cat#65874, 1:2,000) and analyzed using BD flow cytometer.
Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-TANK antibody (Cat#65874, 1:1,000), Top panel: wild-type (WT); Bottom panal: TANK shRNA knockdown (KD). Nuclei were stained blue with DAPI; TANK was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    TANK
  • 货号：
    65874
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-TANK Mouse Monoclonal Antibody
Aliases TANK; TRAF Family Member Associated NFKB Activator; I-TRAF; TRAF2; TRAF Family Member-Associated NF-Kappa-B Activator; TRAF-Interacting Protein; ITRAF; TRAF Family Member-Associated NFKB Activator
Background

Gene Name: TANK

NCBI Gene Entry: 10010

UniProt Entry: Q92844
Application Information

Molecular Weight: Predicted, 48 kDa, observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5605

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human TANK
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
  • Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of TANK knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TANK antibody (Cat#65874, 1:2,000) and analyzed using BD flow cytometer.
  • Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
  • Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using anti-TANK antibody (Cat#65874, 1:1,000), Top panel: wild-type (WT); Bottom panal: TANK shRNA knockdown (KD). Nuclei were stained blue with DAPI; TANK was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    TANK
  • 货号：
    65874
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of TANK knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TANK antibody (Cat#65874, 1:2,000) and analyzed using BD flow cytometer.
Flow cytometric analysis of TANK expression in HepG2 cells using anti-TANK antibody (Cat#65874, 1:2,000). Green, isotype control; red, TANK.
Western blotting analysis using anti-TANK antibody (Cat#65874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TANK antibody (Cat#65874). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK antibody (Cat#65874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-TANK antibody (Cat#65874, 1:1,000), Top panel: wild-type (WT); Bottom panal: TANK shRNA knockdown (KD). Nuclei were stained blue with DAPI; TANK was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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