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KD-Validated Anti-PPP2R1B Mouse Monoclonal Antibody #65875

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Flow cytometric analysis of PPP2R1B expression in HepG2 cells using anti-PPP2R1B antibody (Cat#65875, 1:2,000). Green, isotype control; red, PPP2R1B.
Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). PPP2R1B expression in wild-type (WT) and PPP2R1B shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PPP2R1B
  • 货号:
    65875
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PPP2R1B Mouse Monoclonal Antibody
Aliases PPP2R1B; Protein Phosphatase 2 Scaffold Subunit Abeta; PP2A-Abeta; PR65B; Serine/Threonine-Protein Phosphatase 2A 65 KDa Regulatory Subunit A Beta Isoform; Protein Phosphatase 2 (Formerly 2A), Regulatory Subunit A (PR 65), Beta Isoform; Protein Phosphatase 2, Structural/Regulatory Subunit A, Beta; Protein Phosphatase 2A, Regulatory Subunit A, Beta Isoform; Protein Phosphatase 2, Regulatory Subunit A, Beta; Protein Phosphatase 2 Regulatory Subunit A, Beta; PP2A Subunit A Isoform PR65-Beta; PP2A Subunit A Isoform R1-Beta; PP2A-A-Beta
Background

Gene Name: PPP2R1B

NCBI Gene Entry: 5519

UniProt Entry: P30154

Application Information

Molecular Weight: Predicted, 66 kDa, observed, 62 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5610

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human PPP2R1B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PPP2R1B expression in HepG2 cells using anti-PPP2R1B antibody (Cat#65875, 1:2,000). Green, isotype control; red, PPP2R1B.
  • Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). PPP2R1B expression in wild-type (WT) and PPP2R1B shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of PPP2R1B expression in HepG2 cells using anti-PPP2R1B antibody (Cat#65875, 1:2,000). Green, isotype control; red, PPP2R1B.
Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PPP2R1B antibody (Cat#65875). PPP2R1B expression in wild-type (WT) and PPP2R1B shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R1B antibody (Cat#65875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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