KD-Validated Anti-HSD17B4 Mouse Monoclonal Antibody - 产品中心

KD-Validated Anti-HSD17B4 Mouse Monoclonal Antibody #65880

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Western blotting analysis using anti-HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer.

基因名：

HSD17B4
货号：

65880
应用：

WB, FCM
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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Information

Product Name	KD-Validated Anti-HSD17B4 Mouse Monoclonal Antibody
Aliases	HSD17B4; Hydroxysteroid 17-Beta Dehydrogenase 4; SDR8C1; MFE-2; DBP; 17-Beta-Hydroxysteroid Dehydrogenase 4; 3-Alpha,7-Alpha,12-Alpha-Trihydroxy-5-Beta-Cholest-24-Enoyl-CoA Hydratase; Short Chain Dehydrogenase/Reductase Family 8C Member 1; Peroxisomal Multifunctional Enzyme Type 2; 17beta-Estradiol Dehydrogenase Type IV; Peroxisomal Multifunctional Protein 2; D-Bifunctional Protein, Peroxisomal; D-3-Hydroxyacyl-CoA Dehydratase; Beta-Hydroxyacyl Dehydrogenase; Multifunctional Protein 2; Beta-Keto-Reductase; 17-Beta-HSD IV; 17-Beta-HSD 4; MFP-2; Short Chain Dehydrogenase/Reductase Family 8C, Member 1; Epididymis Secretory Sperm Binding Protein; Hydroxysteroid (17-Beta) Dehydrogenase 4; Hydroxysteroid Dehydrogenase 4; D-Bifunctional Protein; EDH17B4; PRLTS1; MPF-2
Background	Gene Name: HSD17B4 NCBI Gene Entry: 3295 UniProt Entry: P51659
Application Information	Molecular Weight: Predicted, 80 kDa; observed, 73,75 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB5635 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human HSD17B4
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

HSD17B4
货号：

65880
应用：

WB, FCM
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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