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KD-Validated Anti-Bcl-2 Rabbit Monoclonal Antibody #67142

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Flow cytometric analysis of Bcl-2 expression in HT-1080 cells using Bcl-2 antibody (Cat#67142, 1:2,000). Green, isotype control; red, Bcl-2.
Western blotting analysis using anti-Bcl 2 antibody (Cat#67142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bcl-2 antibody (Cat#67142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-2 antibody (Cat#67142, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    BCL2
  • 货号:
    67142
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Bcl-2 Rabbit Monoclonal Antibody
Aliases BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator
Background

Gene Name: BCL2

NCBI Gene Entry: 596

UniProt Entry: P10415

Application Information

Molecular Weight: Predicted, 26 kDa, observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB680

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Bcl-2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Bcl-2 expression in HT-1080 cells using Bcl-2 antibody (Cat#67142, 1:2,000). Green, isotype control; red, Bcl-2.
  • Western blotting analysis using anti-Bcl 2 antibody (Cat#67142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Bcl-2 antibody (Cat#67142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-2 antibody (Cat#67142, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of Bcl-2 expression in HT-1080 cells using Bcl-2 antibody (Cat#67142, 1:2,000). Green, isotype control; red, Bcl-2.
Western blotting analysis using anti-Bcl 2 antibody (Cat#67142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bcl-2 antibody (Cat#67142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#67142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-2 antibody (Cat#67142, 1:2,000) and analyzed using BD flow cytometer.
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