Flow cytometric analysis of alpha smooth muscle Actin expression in HT-1080 cells using alpha smooth muscle Actin antibody (Cat#67172, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.
Immunocytochemical staining of HT-1080 cells with alpha smooth muscle Actin antibody (Cat#67172, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-alpha smooth muscle actin antibody (Cat#67172, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#67172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha smooth muscle Actin antibody (Cat#67172, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#67172). alpha smooth muscle Actin antibody expression in wild type (WT) and alpha smooth muscle Actin antibody shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-alpha smooth muscle Actin antibody (Cat#67172, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of alpha smoothmuscle Actin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-alpha smoothmuscle Actin antibody (Cat#67172, 1:2,000) and analyzed using BD flow cytometer.
