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KD-Validated Anti-Chromodomain helicase DNA binding protein 3 Rabbit Monoclonal Antibody #68323

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Flow cytometric analysis of CHD3 expression in HepG2 cells using anti-CHD3 antibody (Cat#68323, 1:2,000). Green, isotype control; red, CHD3.
Immunocytochemical staining of HepG2 cells with Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000). Nuclei were stained blue with DAPI; Chromodomain helicase DNA binding protein 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Chromodomain helicase DNA binding protein 3 expression in wild type (WT) and chromodomain helicase DNA binding protein 3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Chromodomain helicase DNA binding protein 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000), Top panel: wild-type (WT); Bottom panal: Chromodomain helicase DNA binding protein 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    CHD3
  • 货号：
    68323
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Chromodomain helicase DNA binding protein 3 Rabbit Monoclonal Antibody
Aliases CHD3; Chromodomain Helicase DNA Binding Protein 3; ZFH; Chromodomain-Helicase-DNA-Binding Protein 3; Mi-2 Autoantigen 240 KDa Protein; ATP-Dependent Helicase CHD3; Zinc Finger Helicase; Mi2-ALPHA; Mi-2a; CHD-3; HZFH; Zinc-Finger Helicase (Snf2-Like); EC 3.6.4.12; MI2-ALPHA; Mi2-Alpha; EC 3.6.1; SNIBCPS; MI-2A
Background

Gene Name: CHD3

NCBI Gene Entry: 1107

UniProt Entry: Q12873
Application Information

Molecular Weight: Predicted, 226 kDa, observed, 260 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1305

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human CHD3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CHD3 expression in HepG2 cells using anti-CHD3 antibody (Cat#68323, 1:2,000). Green, isotype control; red, CHD3.
  • Immunocytochemical staining of HepG2 cells with Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000). Nuclei were stained blue with DAPI; Chromodomain helicase DNA binding protein 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Chromodomain helicase DNA binding protein 3 expression in wild type (WT) and chromodomain helicase DNA binding protein 3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Chromodomain helicase DNA binding protein 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000), Top panel: wild-type (WT); Bottom panal: Chromodomain helicase DNA binding protein 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    CHD3
  • 货号：
    68323
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of CHD3 expression in HepG2 cells using anti-CHD3 antibody (Cat#68323, 1:2,000). Green, isotype control; red, CHD3.
Immunocytochemical staining of HepG2 cells with Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000). Nuclei were stained blue with DAPI; Chromodomain helicase DNA binding protein 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323). Chromodomain helicase DNA binding protein 3 expression in wild type (WT) and chromodomain helicase DNA binding protein 3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Chromodomain helicase DNA binding protein 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Chromodomain helicase DNA binding protein 3 antibody (Cat#68323, 1:1,000), Top panel: wild-type (WT); Bottom panal: Chromodomain helicase DNA binding protein 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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